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Category:Peabody Award-winning television programsChiral recognition and interactions of D-amino acids in ion chromatography with modified (zwitterionic) column materials.
In this paper we report on the formation of ion-exchange (IE) stationary phases, modified with (zwitterionic) moieties, that are capable of chiral recognition of D-amino acids. The main point in our investigation was to develop a novel stationary phase, suitable for enantioseparation of D-amino acids, using ion chromatography (IC) as a chromatographic technique. In addition to the introduction of (zwitterionic) moieties to the polymeric backbone of the stationary phase, which provides hydrophilicity, we developed and incorporated a sulfobetaine moiety into the backbone, which is believed to provide the chiral recognition capability of the ion-exchange stationary phases. Two materials were synthesized and evaluated. The resulting chromatographic media were tested for their ability to separate D-enantiomers of amino acids. Chromatographic results were also investigated in terms of retention and selectivity. Chiral recognition behavior of the modified stationary phases were investigated using D-Alanine, D-Aspartic acid, and D-Serine and D-Leucine as the D-amino acid standards, and their racemic and optically pure forms.Q:
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